PB-10 High-pressure freezing method can be replaced by sandwich freezing method !?: electron microscopy of human tissues and cultured cells
نویسندگان
چکیده
منابع مشابه
Tandem high-pressure freezing and quick freeze substitution of plant tissues for transmission electron microscopy.
Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-friendly technique. Traditional sample preparation for TEM used chemical fixatives to preserve cellu...
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We are interested in the mechanisms by which cells generate and maintain their complex architectures. A major structural element in cells are lipid membranes, which define both the external cell shape as well as forming subcellular compartments, such as organelles. Transmission electron microscopy (TEM) remains a method of choice for examine cell ultrastructure, particularly for examining cellu...
متن کاملAn assessment of high-pressure freezing and freeze substitution protocols for cultured cells
Since the advent of cryo-electron microscopy (cryo-EM), the close-to-life and fine structure preservation of biological samples has become a realized procurement, surpassing the fixation quality and avoiding the numerous artifacts inherent to conventional chemical preservation methods. Cryo-EM techniques such as high-pressure freezing followed by freezesubstitution (HPF/FS) have facilitated the...
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In the preservation of tissues in as 'close to life' state as possible, rapid freeze fixation has many benefits over conventional chemical fixation. One technique by which rapid freeze-fixation can be achieved, high pressure freezing (HPF), has been shown to enable ice crystal artefact-free freezing and tissue preservation to greater depths (more than 40 μm) than other quick-freezing methods. D...
متن کاملSelf-pressurized rapid freezing (SPRF): a novel cryofixation method for specimen preparation in electron microscopy.
A method is described for the cryofixation of biological specimens for ultrastructural analysis and immunocytochemical detection studies. The method employs plunge freezing of specimens in a sealed capillary tube into a cryogen such as liquid propane or liquid nitrogen. Using this method a number of single-cell test specimens were well preserved. Also multicellular organisms, such as Caenorhabd...
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ژورنال
عنوان ژورنال: Microscopy
سال: 2019
ISSN: 2050-5698,2050-5701
DOI: 10.1093/jmicro/dfz085